Considerations on Legal and Philosophical Problems in Experimental Botany: The case of plant in vitro cultures


  • Iwona Kleszcz Uniwersytet Jagielloński
  • Marek Jakubiec Uniwersytet Papieski Jana Pawła II w Krakowie


Słowa kluczowe:

experimental botany, in vitro cultures, plant’s axiology, scientific experiments and law


The present paper consists of two parts. In the first, some issues related to the character of biological experiments conducted under in vitro cultures are portrayed. The relevant aspects of these procedures are explicated from the viewpoint of the experimental botanist. It is a case study for the considerations in the second part, which presents selected philosophical and legal issues involved in biological experiments from the general perspective of philosophical investigations concerning the problem of plants’ axiology. Obviously, the nature of the considerations is limited; not all important questions from the perspective of biology and philosophy have been raised. Nevertheless, the authors hope that the analyzed issues may be interesting for both biologists and philosophers.

Biogramy autorów

  • Iwona Kleszcz - Uniwersytet Jagielloński
    Doktorantka w Zakładzie Cytologii i Embriologii Roślin Instytutu Botaniki Uniwersytetu Jagiellońskiego. W swoich badaniach poświęca szczególną uwagę kwestiom związanym z roślinnymi kulturami in vitro, szczególnie w kontekście możliwości regeneracyjnych bielma. Obecnie pracuje na kulturach in vitro kiwi, różnych odmian pszenicy i pszenżyta, a wkrótce także słonecznika. Poza zagadnieniami związanymi z roślinami i ich rozwojem interesuje się tematyką dydaktyki szkolnej i akademickiej.
  • Marek Jakubiec - Uniwersytet Papieski Jana Pawła II w Krakowie
    Absolwent studiów filozoficznych I i II stopnia na Uniwersytecie Papieskim Jana Pawła II w Krakowie i prawa na Uniwersytecie Jagiellońskim; doktorant na Wydziału Prawa i Administracji Uniwersytetu Jagiellońskiego oraz Wydziału Filozoficznego Uniwersytetu Papieskiego Jana Pawła II w Krakowie. Zajmuje się głównie filozofią prawa, filozofią języka i filozofią nauki.


Adamczuk A., Siegień I., Ciereszko I., Morphogenesis of Plants in vitro under Stress Conditions, [in:] Biological Diversity – from Cell to Ecosystem, ed. G. Łaska, Białystok 2012.

Agrawal A. A., Communication between Plants: this Time It’s Real, “Trends in Ecology & Evolution” vol. 15 (2000).

How Rapid is Aphid-Induced Signal Transfer Between Plants via Common Mycelial Networks?, “Communicative & Integrative Biology” vol. 6 (2013) 6, no. e25904.

Babikova Z., Gilbert L., Bruce T., Birkett M, Caulfield J. C., Woodcock Ch., Pickett J., Johnson D., Underground Signals Carried through Common Mycelial Networks Warn Neighbouring Plants of Aphid Attack, “Ecology Letters” vol. 16 (2013), p. 835–843.

Bach A., Pawłowska B., Procesy rozwojowe w kulturach in vitro i typy kultury, [in:] Biotechnologia roślin, red. S. Malepszy, Warszawa 2009.

High-Tech and Micropropagation V, ed. Y. P. S. Bajaj, New Delhi 1997a (Biotechnology in Agriculture and Forestry, 39).

High-Tech and Micropropagation V, ed. Y. P. S. Bajaj, New Delhi 1997b (Biotechnology in Agriculture and Forestry, 40).

Bajaj Y. P. S., In vitro Production of Haploids and Their Use in Cell Genetics and Plant Breeding, “Biotechnology in Agriculture and Forestry” vol. 12 (1990), p. 3–44.

Baldwin I. T., Plant Volatiles, “Current Biology” vol. 20 (2010), p. R392–R397.

Ball E., Development in Sterile Culture of Stem Tips and Subjacent Regions of Tropaeolum majus L. and of Lupinus albus L., “American Journal of Botany” vol. 33 (1946) 5, p. 301–318.

Bhojwani S. S., Razdan M. K., Plant Tissue Culture: Theory and Practice, a revised edition, New Delhi 2004.

Cassells A. C., Doyle B. M., Pathogen and Biological Contamination Management: the Road Ahead, [in:] Plant Cell Culture Protocols, ed. V. M. Loyola-Vargas, F. Vázquez-Flota, New York 2005, p. 35–50.

Christianson M. L., Warnick D. A., Temporal Requirement for Phytohormone Balance in the Control of Organogenesis in vitro, “Developmental Biology” vol. 112 (1985), p. 494–497.

Dagla H. R., Plant Tissue Culture. Historical Developments and Applied Aspects, “Resonance” vol. 17 (2012) 8, p. 759–767.

Dudley S. A., File A. L., Kin Recognition in an Annual Plant, “Biology Letters” vol. 3 (2007), p. 435–438.

Eady C. C., Butler R. C., Suo Y., Somatic Embryogenesis and Plant Regeneration from Immature Embryo Cultures of Onion (Allium cepa L.), “Plant Cell Reports” vol. 18 (1998) 1–2, p. 111–116.

Engelmann F., Engels J. M. M., Technologies and Strategies for ex situ Conservation, [in:] Managing Plant Genetic Diversity, ed. J. M. M. Engels, R. Rao, A. H. D. Brown, M. T. Jackson, Oxford 2002, p. 89–104.

Falik O., Mordoch Y., Ben-Natan D., Vanunu M., Goldstein O., Novoplansky A., Plant Responsiveness to Root–Root Communication of Stress Cues, “Annals of Botany” vol. 110 (2012), p. 271–28.

Falik O., Mordoch Y., Quansah L., Fait A., Novoplansky A., Rumor Has It…: Relay Communication of Stress Cues in Plants, “PLoS ONE” vol. 6 (2011) 11, no. e23625.

Gonzalez-Arnao M. T., Panta A., Roca W. M., Escobar R. H., Engelmann F., Development and Large Scale Application of Cryopreservation Techniques for Shoot and Somatic Embryo Cultures of Tropical Crops, “Plant Cell, Tissue and Organ Culture (PCTOC)” vol. 92 (2008) 1, p. 1–13.

Goren R., Altman A., Giladi I., Role of Ethylene in Abscisic Acid-Induced Callus Formation in Citrus Bud Cultures, “Plant Physiology” vol. 63 (1979), p. 280–282.

Gorst J. R., The Concept of Totipotency, [in:] Plants in Action. Adaptation in Nature, Performance in Cultivation, ed. B. J. Atwell, P. E. Kriedemann, C. G. N. Turnbull, Melbourne 1999,–2-1-concept-totipotency (17.03.2015).

Hall M., Plants as Persons. A Philosophical Botany, Albany (NY) 2011.

Haq I., Chang-Xing Z., Mukhtar Z., Jaleel C. A., Azooz M. M., Effect of Physical Desiccation on Plant Regeneration Efficiency in Rice (Oryza sativa L.) Variety super basmati, “Journal of Plant Physiology” vol. 166 (2009).

Harrison J. H., Foreword, [in]: Experiment in Plant Tissue Culture, ed. J. H. Dodds, L. W. Roberts, New York 1985.

Ikeuchi M., Sugimoto K., Iwase A., Plant Callus: Mechanisms of Induction and Repression, “The Plant Cell”, vol. 25 (2013), p. 3159–3173.

Karban R., Baldwin I. T., Baxter K. J., Laue G., Felton G. W., Communication between Plants: induced Resistance in Wild Tobacco Plants following Clipping of Neighboring Sagebrush, “Oecologia” vol. v. 125 (2000), p. 66–71.

Karpiński S., Reynolds H., Karpińska B., Wingsle G., Creissen G., Mullineaux P., Systemic Signaling and Acclimation in Response to Excess Excitation Energy in Arabidopsis, “Science” vol. 284 (1999) 5414, p. 654–657.

Khanna H. K., Daggard G. E., Enhanced of Shoot Regeneration in Nine Australian Wheat Cultivars by Spermidine and Water Stress Treatments, “Australian Journal of Plant Physiology” vol. 28 (2001), p. 1243–1247.

Kozai T., Kubota C., Jeong B. R., Environmental Control for the Large-Scale Production of Plants through in vitro Techniques, “Plant Cell, Tissue and Organ Culture (PCTOC)” vol. 51 (1997) 1, p. 49–56.

Marcińska I., Nowakowska A., Skrzypek E., Czyczyło-Mysza I., Production of Double Haploids in Oat (Avena sativa L.) by Pollination with Maize (Zea mays L.), “Central European Journal of Biology” vol. 8 (2013) 3, p. 306–313.

Marder M., Should Plants Have Rights?, “The Philosopher's Magazine” 62 (2013).

Matysiak K., Adamczewski K., Regulatory wzrostu i rozwoju roślin – kierunki badań w Polsce i na świecie, „Progress in Plant Protection” vol. 49 (2009) 4, p. 1810–1816.

McCabe P. F., Leaver C. J., Programmed Cell Death in Cell Cultures, “Plant Molecular Biology” vol. 44 (2000), p. 359–268.

Murashige T., Skoog F., A Revised Medium for Rapid Growth and Bio-Sssays with Tobacco Tissue Cultures, “Physiologia Plantarum”, vol. 15 (1962), p. 473–497.

Murphy G.P., Dudley S. A., Kin Recognition: Competition and Cooperation in Impatiens (Balsaminaceae), “American Journal of Botany” vol. 96 (2009), p. 1990–1996.

Nagata T., Takebe I., Plating of Isolated Tobacco Mesophyll Protoplasts on Agar Medium, “Planta” vol. 99 (1971), p. 12–20.

Phillips G. C., In vitro morphogenesis in plants – recent advances, “In Vitro Cellular & Developmental Biology – Plant” vol. 40 (2004), p. 342–345.

Popielarska-Konieczna M., Kozieradzka-Kiszkurno M., Tuleja M., Ślesak H., Kapusta P., Marcińska I., Bohdanowicz J., Genotype-Dependent Efficiency of Endosperm Development in Culture of Selected Cereals: Histological and Ultrastructural Studies, “Protoplasma” vol. 250 (2013) 1, p. 361–369.

Puijalon S., Piola F., Bornette G., Abiotic Stresses Increase Plant Regeneration Ability, “Evolutionary Ecology” vol. 22 (2008), p. 493–506.

Jain R. K., Jain S., Wu R., Stimulatory Effect of Water Stress on Plant Regeneration in Aromatic Indica Rice Varieties, “Plant Cell Reports” vol. 15 (1996), p. 449–454.

Rout G. R., Samantaray S., Das P., In vitro Manipulation and Propagation of Medicinal Plants, “Biotechnology Advances”, vol. 18 (2000) 2.

Schleiden M. J., Beiträge zur Phytogenesis, Berlin 1838 (Archiv für Anatomie, Physiologie und Wissenschaftliche Medicin, von. J. Müller).

Schwann T., Mikroscopische Untersuchungen über die Übereinstimmung in der Struktur und dem Wachstum des Thiere und Pflanzen, von W. Engelmann, Leipzig 1839.

Plant Propagation by Tissue Culture, 3rd Edition, Volume 1. The Background, ed. E. F. George, M. A. Hall, G.-J. de Klerk, The Netherlands 2008.

Skoog F., Miller C. O., Chemical Regulation of Growth and Organ Formation in Plant Tissues Cultured in vitro, “Symposia of the Society for Experimental Biology” vol. 11 (1957), p. 118–130.

Ślesak I., Libik M., Karpińska B., Karpiński S., Miszalski Z., The Role of Hydrogen Peroxide in Regulation of Plant Metabolism and Cellular Signalling in Response to Environmental Stresses, “Acta Biochimica Polonica” vol. 54 (2007), p. 39–50.

Steward F. C., Mapes M. O., Mears K., Growth and Organized Development of Cultured Cells. II. Organization in Cultures Grown from Freely Suspended Cells, “American Journal of Botany” vol. 45 (1958), p. 705–708.

Stone C. D., Environmental Legal Rights, „Southern California Law Review” 45 (1972), pp. 456–457.

Sussex M., The Scientific Roots of Modern Plant Biotechnology, “The Plant Cell” vol. 20 (2008) 5, p. 1189–1198.

Szechyńska M. Szechyńska-Hebda, J. Kruk, M. Górecka, B. Karpińska, S. Karpiński, Evidence for Light Wavelength-Specific Photoelectrophysiological Signaling and Memory of Excess Light Episodes in Arabidopsis, “The Plant Cell” vol. 22 (2010) 7, p. 2201–2218.

Taylor P., Respect for Nature, Princeton (NJ) 2011.

Tretyn A., Podstawy strukturalno-funkcjonalne komórki roślinnej, [in:] Fizjologia roślin, red. J. Kopcewicz, S. Lewak, Warszawa 2012, p. 22–84.

Yu C., Chen Z., Lu L., Lin J, Somatic Embryogenesis and Plant Regeneration from Litchi Protoplasts Isolated from Embryogenic Suspensions, “Plant Cell, Tissue and Organ Culture (PCTOC)” vol. 61 (2000) 1, p. 51–58.

Vasil I. K., A History of Plant Biotechnology: from the Cell Theory of Schleiden and Schwann to Biotech Crops, “Plant Cell Reports” vol. 27 (2008) 9, p. 1423–1440.

Zebelo S. A., Maffei M. E., Role of Early Signalling Events in Plant-Insect Interactions, “Journal of Experimental Botany” vol. 66 (2015) 2, p. 435–448.

Zenkteler M., Zenkteler E., 65 years of in vitro Culture in Poland, “Acta Societatis Botanicorum Poloniae”, vol. 82 (2013) 3, p. 183–192.